DNA Microarray and insitu analysis of gene expression in Drosophila embryos.

Microarray analysis : Wild type embryos were collected in 1 hour intervals spanning the first 12 hours of Drosophila embryogenesis. Each collection was repeated three times independently. Total RNA was isolated using Ambion's RNAwiz and labelled using standard Affymetrix Gene Chip protocols and hybridised to Drosophila Gene Chip arrays. Data were analysed by Affmetrix Microarray Suite 4.0 and dChip algoritm.

RNA insitu hybridisation : RNA insitu hybridisation in 96 well format was performed as described (link). Embryos collections were biased towards early stages of embryogenesis when gap and pair-rule patterning processes occur. Images were recorded using Zeiss Axiophot light microscope and stored in a mySQL database.

The following table summarizes available insitu and microarray data for all genes from Supplemental Table 2

Nr. CG name Flybase name Nr_of_images Details
1 CG7952 gt  19 images and arrays
2 CG7925 tko  0 array data
3 CG12688 CG12688  0 array data
4 CG3011 CG3011  0 array data
5 CG3726 CG3726  7 images and arrays
6 CG9650 CG9650  10 images and arrays
7 CG1958 CG1958  0 array data
8 CG11368 CG11368  0 array data
9 CG11369 CG11369  0 array data
10 CG12154 oc  27 images and arrays
11 CG12109 Caf1-180  0 array data
12 CG12653 btd  20 images and arrays
13 CG1343 Sp1  0 array data
14 CG5291 CG14415  0 array data
15 CG18313 CG18313  0 array data
16 CG3851 CG3851  72 images and arrays
17 CG2788 Dot  0 array data
18 CG6246 nub  45 images and arrays
19 CG15488 CG15488  0 array data
20 CG12287 pdm2  5 images and arrays
21 CG15486 CG15486  0 array data
22 CG4501 bgm  0 array data
23 CG8930 rk  9 images and arrays
24 CG5803 Fas3  0 array data
25 CG10305 RpS26  0 array data
26 CG2368 psq  49 images and arrays
27 CG5580 sbb  79 images and arrays
28 CG14502 CG14502  31 images and arrays
29 CG7097 CG7097  0 array data
30 CG13534 CG13534  0 array data
31 CG9898 CG9898  0 array data
32 CG14178 CG14178  42 images and arrays
33 CG17357 CG17357  0 array data
34 CG17705 ome  7 images and arrays
35 CG13473 CG13473  0 array data
36 CG4319 rpr  25 images and arrays
37 CG4345 grim  0 array data
38 CG8786 CG8786  21 images and arrays
39 CG8782 CG8782  0 array data
40 CG2189 Dfd  24 images and arrays
41 CG2198 Ama  0 array data
42 CG1028 Antp  43 images and arrays
43 CG2047 ftz  15 images and arrays
44 CG9755 pum  31 images and arrays
45 CG18516 CG18516  0 array data
46 CG5302 CG5302  0 array data
47 CG5557 sqz  10 images and arrays
48 CG14282 CG14282  0 array data
49 CG5320 Gdh  21 images and arrays