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User-defined motifs can be specified either by entering a collection of example sequences (formats 1-2) or by entering a frequency matrix (format 3) into the fields below. You can also upload text files using these same formats.
Note: Searches will be very slow the first time you search using a new motif, but the motif will then be cached and subsequent runs will be normal speed.
All three examples below correspond to the same binding motif (Knirps motif as described in Small, et. al., Dev Biol 1996). Key aligned residues are in red.
> seq1 AACTAGATCA > seq2 AAAGAGAGCA > seq3 AACGCGGCCA > seq4 AAGTAGATCA > seq5 AACTAGCGCA
> seq1 AGTAAACTAGATCAGCC > seq2 AAAGAGAGCAAG > seq3 GAACGCGGCCAA > seq4 AAGTAGATCA > seq5 AACTAGCGCAATTT
A| 5 5 1 0 4 0 3 0 0 5 C| 0 0 3 0 1 0 1 1 5 0 T| 0 0 0 3 0 0 0 2 0 0 G| 0 0 1 2 0 5 1 2 0 0